Catalog Number - 75001-05P

75001-05P Cogent HPS Silica™ HPLC Column is packed with a high‑purity, unmodified silica stationary phase, offering reliable and reproducible performance for normal phase chromatography and USP L3 classified methods.

Because the silica is not chemically modified, it provides strong interaction with polar and hydrophilic compounds, making it ideal for separations involving fats, lipids, and other polar analytes.

This proprietary HPS Silica™ has been widely adopted in analytical laboratories as a dependable and cost‑effective alternative to traditional silica columns such as Inertsil® and Luna®, delivering consistent selectivity and performance.

The 5 µm particle size ensures a balance of efficiency and durability, allowing operation at higher flow rates with lower backpressure, making it well suited for routine analytical methods on standard HPLC systems.

50 mm Length – Fast Normal Phase Separations

The 50 mm column length is optimized for speed and throughput.

• Shorter run times for rapid analysis

• Reduced solvent consumption

• Ideal for method development and screening

✅ Recommended for high‑throughput normal phase HPLC workflows

4.6 mm ID – Standard Analytical HPLC Format

• Designed for 4.6 mm ID analytical columns

• Robust and widely compatible with standard HPLC systems

• Higher sample loading vs narrower ID columns

• Well suited for routine analytical methods

✅ Recommended for QC labs and general-purpose analytical HPLC

Flow Rate & System Requirements (4.6 mm ID, 5 µm)

✅ Compatible with standard HPLC systems

• Typical flow rate range: ~1.0 – 1.5 mL/min

• Lower backpressure vs smaller particle columns

• Suitable for routine analytical separations

• Compatible with UV and other standard detectors

✅ Best Practice: Optimize mobile phase composition for normal phase retention and reproducibility

Why Choose HPS Silica™ Columns

High‑Purity Unmodified Silica

• Ideal for normal phase chromatography

• Strong retention of polar compounds

USP L3 Compatibility

• Suitable for compendial methods

• Reliable alternative to legacy silica columns

Consistent Performance

• Reproducible selectivity

• Proven long-term reliability

Performance Advantages

Low Backpressure (5 µm Particles)

• Enables higher flow rates

• Compatible with conventional systems

Fast Equilibration

• Reduces downtime between runs

Excellent for Polar Analytes

• Ideal for lipids, fats, and hydrophilic compounds

Typical Applications

• Fats and lipid analysis

• Normal phase HPLC separations

• Polar compound analysis

• USP L3 method applications

• Food, pharmaceutical, and chemical testing

Important Usage Notes

• Designed for normal phase chromatography conditions

• Use appropriate non‑polar mobile phases (e.g., hexane-based systems)

• Ensure proper system equilibration for reproducible retention

• Follow standard silica column handling procedures